Biotech Spain  Techniques

X-ray crystallography

17/01/2014 Last update:17/01/2014

X-ray crystallography allows to determine the structure of biological molecules with high resolution.

Eukaryotic cell culture

22/03/2012 Last update:17/06/2013

Cell cultures are at the core of a large part of basic and applied research. Characterizing the function of new genes and proteins would not be possible without resorting to cell-based in vitro assays at some point. Small- and large-scale protein production and generation of monoclonal antibodies are equally based on cell culture at least in some stages of their process. In addition, many varieties of transgenic animals and plants often start from cell cultures.

Electrophoresis: DNA and proteins in a race

30/09/2011 Last update:30/09/2011

Electrophoresis is perhaps the technique most used in laboratories to sort complex mixtures of DNA fragments or proteins and classify them according to their size.

Conditional knockouts

24/06/2011 Last update:24/06/2011

One way to study the evolution of a disease implies using an animal model that mimics human disease as closely as possible. In the case of those diseases caused by the failure of a particular cell type or tissue, researchers create animal models with genetic defects connected to that mistake. These are known as knockout mice.

Optogenetics: how to activate cells turning on the light

17/05/2011 Last update:17/05/2011

By means of optogenetics, we can use light to control cell activation. The use of light as inducing agent makes cell response extremely rapid and selective

Flow cytometry: measuring cells.

25/04/2011 Last update:25/04/2011

Flow cytometry is a powerful technique that allows multiparametric analysis in individual cells starting from heterogeneous populations.

Synthetic biology and its applications

24/02/2011 Last update:29/04/2013

Synthetic biology brings together engineers and biologists when it comes to the design and construction of biomolecular components, networks and metabolic pathways from scratch, using these constructs to reprogram organisms. This de novo engineering of genetic circuits, biological modules and synthetic pathways is beginning to solve critical issues in biology and is already being used in practical applications.

Protein engineering using circular permutation.

17/01/2011 Last update:01/02/2011

Circular permutation is a strategy naturally followed by evolution to create new proteins from already existing ones and which can be used in research to obtain proteins with enhanced functionalities or new features.

Expression systems for recombinant proteins (II). Producing organisms.

28/09/2010 Last update:25/04/2011

Nowadays, it is possible to synthesize recombinant proteins from animals and plants, both prokaryotic and eukaryotic. Recombinant proteins can be expressd in cultures from bacteria, yeast, fungi, mammal, plant and insect cells.Transgenic plants and animals have also been developed for the production of relevant proteins.

Recombinant proteins expression systems (I). Genes and vectors

28/09/2010 Last update:28/02/2011

Proteins differ from traditional industrial chemicals in their synthesis system. The vast majority of these proteins with a specific action are recombinant, which means the protein synthesis machinery in a specific organism is used to produce them. We will analyze the main aspects to be considered in order to express a recombinant protein, by looking at the key points that determine the final outcome.